RNA
Part:BBa_K3001025
Designed by: Sydnee Calhoun Group: iGEM19_Lethbridge_HS (2019-10-19)
snR30 + RNA Mango II
Purification tag for the RNA cleaving enzyme Cas13a isolated from Leptotrichia buccalis . Contains a His tag, Twin strep tag, and a SUMO tag. Please see part BBa_K3001028 for experience using this part.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 594
Illegal SpeI site found at 134
Illegal PstI site found at 1053 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 529
Illegal SpeI site found at 134
Illegal PstI site found at 1053 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 546
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 594
Illegal SpeI site found at 134
Illegal PstI site found at 1053 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 594
Illegal SpeI site found at 134
Illegal PstI site found at 1053 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1024
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Categories
Parameters
None |